Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
نویسندگان
چکیده
منابع مشابه
Image analysis of breast cancer immunohistochemistry-stained sections using ImageJ: an RGB-based model.
BACKGROUND Image analysis of tissue sections using RGB image profiling is a modern accepted technique. MATERIALS AND METHODS A new method of RGB analysis, using the freeware ImageJ, is presented which can be applied to sections with either nuclear or cytoplasmic staining. The step-by-step process is presented and the method is tested using breast cancer specimens immunostained for CK-19 and e...
متن کاملP9: Isolation and Culture of Adult Microglia Cells Derived from Epileptic Human Brain Tissue
لطفاً به چکیده انگلیسی مراجعه شود.
متن کاملPrimer for immunohistochemistry on cryosectioned rat brain tissue: example staining for microglia and neurons.
Immunohistochemistry is a widely used technique for detecting the presence, location, and relative abundance of antigens in situ. This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brain tissue, using markers for microglia and neuronal elements as an example. Specifically, this paper is a step-by-step pr...
متن کاملVisual programming of ImageJ using ImageFlow
ImageFlow is a plugin that allows the generation and execution of macros in ImageJ based on the visual programming paradigm. Image processing steps are described by connected nodes on a workspace. Specifying image-processing algorithms corresponds to visually editing block diagrams (the workflow of successive processing steps). This abstraction makes it very flexible and easy to save, restore a...
متن کاملFixation and immunohistochemistry of lymphoid tissue.
Jacobsen CF, Leonis J, Linderstr0m-Lang K, Ottsen M. The pH-stat and its use in biochemistry. Methods Biochem Anal 1957;4:171-210. 2 Heller M, Hanahan DJ. Human erythrocyte membrane bound enzyme: acetylcholinesterase. Biochim Biophys Acta 1972;255:251-72. 3 Newman PFJ. A study of acetylcholine acetyl-hydrolase in the human erythrocyte. MPhil thesis, Edinburgh University, 1974. 4 Baggott J. The ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2018
ISSN: 1940-087X
DOI: 10.3791/57648